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We routinely pick a total of 192 clones (or two 96 well blocks) per viral sample for bidirectional sequencing as this represents the most affordable sequence coverage to efficiency ratio.
PCR approaches are practically restricted to tiny fractions, and next-generation sequencing (NGS) approaches of whole individual genomes e.g. by the 1000 Genomes Project is confined by an affordable sequence depth.
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Recent advances in DNA sequencing have enabled the rapid and affordable sequencing of actinomycete genomes and revealed a large number of secondary metabolite gene clusters with no known products.
The advent of faster and more affordable sequencing technology, coupled with increased computational power, has permitted comparisons of whole Listeria genome sequences from isolates recovered from clinical, food, and environmental sources.
This raises the question if the shorter reads obtained with the more affordable sequencing technologies are sufficient for gene expression studies – i.e.: could they be mapped to the corresponding genes with a similar efficiency as longer sequence reads?
Their increasing availability is due to outstanding advances in the development of fast, efficient and affordable sequencing technologies [ 1].
The advent of affordable sequencing technology provides for a new generation of explorers who probe the world's microbial diversity.
Over the past decade, next-generation sequencing (NGS) has developed to the point that rapid and relatively affordable sequencing of individual genomes is a reality [ 10].
Ion Torrent's semiconductor sequencing technology, as implemented in its Personal Genome Machine (PGM), has been gaining popularity as a fast and affordable sequencing platform since it's debut in 2011 (Merriman et al., 2012; Rothberg et al., 2011).
Until whole genome sequencing for large cohorts becomes affordable sequencing "extended" exomes seems to provide a sensible way to reduce costs while maximizing the chances of detecting disease-associated variants.
As whole-genome sequencing becomes more affordable and sequence analysis more efficient, these tools will greatly aid in refining epidemiological investigations.
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