Paraffin blocks were sectioned and the sections affixed to glass slides.
The fixator construct is made up of two sections: one applied to the tibia and a foot section affixed to the hindfoot, midfoot and forefoot parts.
If you go to the online comment section affixed to Morgenson's article, you see advocates of these two positions talking past one another, one side talking the morality of social protection and the other the morality of personal responsibility.
Ms. Ono gave Mr. Scardino two of them, "India, India" (which now accompanies a scene about the Beatles' flirtation with Transcendental Meditation) and "I Don't Want to Lose You," affixed to the section about Lennon's 18-month separation from Ms. Ono starting in 1973.
One section was affixed to a standard glass slide, and then stained with hematoxylin and eosin (H&E).
Cell aggregates were washed in PBS, fixed in 4% PFA for 30 minutes, washed twice in PBS and embedded in paraffin wax. 5 µm microtome sections were affixed to Poly-L-Lysine coated slide (ProSciTech), dewaxed using Histoclear, rehydrated through an ethanol/H2O gradient and stained with hematoxylin and eosin (H&E).
Paraffin, coronal 5 μm sections were affixed to Fisher brand Superfrost/Plus slides to ensure adhesion.
The sections were affixed to Superfrost Plus slides (CML, Nemours, France), deparaffinised, and rehydrated.
Five micrometer thick sections were affixed to slides, deparaffinized, and stained with hematoxylin and eosin.
Sections were affixed to slides by using Permount histologic mounting medium (Fisher, Fair Lawn, NJ, USA), and coverslips were placed.
Histological sections were cut at 4 μ and stained with hematoxylin and eosin (H&E) stains and additional sections were affixed to charged slides for IHC.
