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PCL nanofiber coated TCPS was then placed in a vacuum overnight to insure removal of residual solvent [ 44], and then plasma treated (Harrick Plasma) to promote cellular attachment [ 36], and subsequently affixed to 24-well cell culture plates for cellular experiments.
Sections were taken every 7 µm, but only sections from 150 µm to 550 µm were subsequently affixed to glass slides for staining as these contained the articular cartilage.
Subsequently, cells were washed twice in PBS and affixed to Superfrost (Fisher) microscope slides with 1 2 drops of SlowFade Gold (Invitrogen) to minimize loss of fluorescent signal.
Images were subsequently opened in the Metamorph software and a grid was affixed on the image defining three regions of interest (ROIs).
Hyphens indicate affix attachment points.
Instead, affix them to your head.
"Affix ordering in Optimal Construction Morphology".
Let the lamp affix its beam.
Now you may affix your stares squarely back on Dallas.
The feature set is augmented with the resulting affix matches.
One is to affix a loud alarm to the vehicle.
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CEO of Professional Science Editing for Scientists @ prosciediting.com