The phrase "affinity without" is correct and usable in written English
It means a strong liking or connection for something or someone, without any other elements or factors influencing it. Example: "Samantha's affinity for nature was evident from a young age, as she spent hours exploring the woods and studying different plant species without any external encouragement."
The pKM19 plasmid facilitates an efficient maturation process, resulting in selection of antibodies with improved affinity without any stop codons.
Computational structural biology tools allowed for the design of rational mutations in the anti-CD123 CAR antigen binding domain that altered CAR expression and CAR binding affinity without affecting the overall CAR design.
Through the combination of single-peptoid mimics and thio-ether bridged cyclization, we successfully demonstrated for the first time two cyclic peptomers, PL-116 and PL-120, dramatically improved the binding affinity without losing mono-specificity against Plk1 PBD in comparison with the linear parental peptide, PLHSpT.
Nevertheless, the possibility that genes encoding these receptors could carry mutations affecting toxin affinity without affecting their expression cannot be discarded with our analyses.
This ensures better binding affinity without going through the SELEX reiterative processes.
Then they got high-binding affinity without chemotactic activity product, named CXCL8(3–72) K11R/G31P (G31P in abbreviation).
Since RNase treatment significantly enhanced gemin4 recovery by cap-affinity without affecting gemin5 pull-down, we reasoned that gemin4 isolation was secondary to its binding partner gemin5, and thus focused our study on the latter.
In order to measure comparable binding affinities without avidity effects resulting from different oligomeric states (BMP-2 as homodimer will bind to two receptor molecules simultaneously if used as analyte) we immobilized both the monovalent Fabs AbD1556 and AbD1564 (another neutralizing anti-BMPR-IA Fab) as well as BMP-2 in different flow cells of one biosensor chip.
A novel purification protocol has been designed to purify the fusion proteins using metal affinity chromatography, without the need of a hexahistidine-tag.
MASS-1 sensitivity was independent of protein immobilization level and kinetic analysis performed using ultra-low density mAb surfaces allowed characterization of picomolar affinity interactions without mass transport limitation.
Recombinant protein purification was conducted using two different strategies, ion exchange and affinity chromatography, without fusion tags.
