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As such, an efficient single step affinity process to purify recombinant proteins from serum-containing medium was optimized.
But ITC cannot study very high and low affinity process and the experiments that need a great deal of accurate measurements.
Gossypetin suppressed the oxidation of LDL [ 143] and was able to modify the LDL in a form accepted by macrophage through elevated affinity process in a nonoxidative mechanism [ 154].
The most common example of an affinity process is protein-A chromatography, which has been applied for over a decade in industrial and academic settings for the capture and purification of antibodies (immunoglobulins) [ 23].
We observe very good agreement between predictions and experimental data for the spatiotemporal behavior of the coupled ITP and affinity process, and for key figures of merit, including scaled capture length and maximum capture efficiency.
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We present immunization strategies that attempt to recapitulate these natural processes and guide the affinity maturation process.
However, sorption in the pH range 2 7 might be an affinity sorption process.
These variants were taken from different steps of an affinity maturation process.
Previously, a 3-million-fold affinity maturation process was achieved in a protein protein interaction composed of a variant T-cell receptor fragment and a bacterial superantigen.
An experimental model of affinity filtration process was designed using a macroligand composed by Cibacron Blue F3GA immobilized to yeast cells.
Here, we propose a concept for improving the ligand-binding properties of this anticalin in an in vitro affinity maturation process by step-wise randomization of restricted areas of the loop region.
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