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The CB2 binding affinity assay as well as functional assay was also conducted on these compounds.
By implementing an affinity assay based on the competitive bonding between concanavalin A and dextran, the selectivity of the membrane can be transferred to the glucose specific recognition of the affinity assay.
After SPR-based binding affinity assay, 15 compounds were found to show binding affinities to CypA at submicro-molar or micro-molar level (compounds 1 15).
A high-throughput melanin chromatographic affinity assay has been developed and validated, which has allowed the rapid melanin affinity assessment for a large number of compounds.
Seven compounds displayed much more potent activity than the reference drug, AMD3100, in both the binding affinity assay and the blocking of Matrigel invasion functional assay.
Further the viscous matrix of the affinity assay prevented the efflux of assay components that was observed using individual molecules such as albumin.
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Study design: Apoptosis in aspirated granulosa cells (n=64) was measured using the Annexin V-affinity assay by flow cytometry.
The remaining mixtures were then purified using the GST-affinity assay as described above.
For Cap-affinity assay we followed the protocol described previously [11].
To determine whether zeolin was associated with folding chaperones, an ATP-affinity assay was performed on crude proteins extracted from transgenic storage roots.
Results from the cap-affinity assay show that the quantity of eIF4E bound to 4E-BP1 inversely correlates with the level of eIF4G bound to eIF4E.
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