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Poor resolution affects quantification of myocardial activity and IDIFs.
The spatial resolution of the imaging system also affects quantification due to the partial volume effect, particularly in the case of small animals.
Often, both process knowledge and data are subject to uncertainty that affects quantification of land use systems in their inputs and outputs.
PCR amplification bias significantly affects quantification accuracy, because final sequence read counts may not accurately represent the relative abundance of original DNA and RNA fragments.
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The main imaging factor affecting quantification, however, remains the partial volume effect (PVE).
The variability of many imaging aspects that affect quantification (e.g. [6, 38]) must also be considered and standardised in wider applications of this method to estimate heterogeneity.
The best choice of PSF reconstruction parameters will be a trade-off between lesion detectability and avoiding PSF artifacts affecting quantification, which should be optimized for the specific task.
Amounts of DNA used (300 ng or 1000 ng DNA) for droplet digital PCR (ddPCR) had an effect on the occupancy of the reference genes (PCR positive droplet count); but the variation observed in occupancy did not affect quantification of the spiked samples.
However, transcription of the Otx2 locus after self-knockout was not affected: quantification of deleted and full length mRNAs by RT-qPCR using exon 3 primers (Fig. 1C) and Cre mRNA by in situ hybridization (Fig. S3, right panel) revealed a steady-state level of mRNA, ruling out Otx2 autoregulation in the mature retina.
However, surface roughness can affect quantification accuracy.
PVE does indeed affect quantification of tumour uptake causing an increasing negative bias in smaller tumours.
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