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Variation in cell wall composition predicted by the genome of W. dermatitidis likely affects interactions with the host immune system.
To place our findings in context with previous reports demonstrating that the valency of Fc-containing compounds affects interactions with their cognate receptors [ 16], we evaluated binding of the individual fractions of 2A-2HC with FcγRIIb, FcγRIII, and SIGN-R1 by SPR, and with FcγRIIb and FcγRIII by Biolayer Interferometry (see Additional File 1, Figure S1).
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Interestingly, deletion analysis of the CPEB3 prion domain uncovered a tripartite organization: two aggregation-promoting domains surround a regulatory module that affects interaction with the actin cytoskeleton.
The heparin-binding motif of IGFBP-5 affects interaction with glycosaminoglycan components of the ECM.
The introduced helix constraints limit the conformational flexibility of the peptides without affecting interactions with 4E10.
Recent applications of this approach to both potassium channels [9] and voltage-gated calcium channels [10] has identified a number of new mutations that affect interactions with channel modifiers [4].
DGCR8 phosphorylation does not appear to significantly affect interactions with Drosha.
We hypothesised that the PY motif of RNF11 could affect interactions with Smad7.
Changes affecting interactions with upstream activators such as ATM, for example, may not be detectable by our measure.
If a parent is suffering from PND, it may affect interactions with not only the baby but the entire family.
Likewise, retraction of the important Ub C-terminus would affect interactions with most enzymes in the Ub system.
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