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Delivering sufficient oxygen to the transplanted cells is one of the most critical issues that affects cell survival and correct maturation of engineered tissues.
To confirm that apoEdp affects cell survival, cell viability was determined using the cell titer 96®AQuous One solution cell proliferation kit.
To test whether expression of the vinculin-ΔEx20 variant affects cell survival during development, TUNEL-staining on histological sections from E10.5 embryos was performed.
We then extended these studies to consider if loss of ALKBH5 during hypoxia/reoxygenation affects cell survival by carrying out clonogenic survival assays (Supplementary Figure S3).
To determine how IL-21 affects cell survival in the presence of pro-survival cytokines we performed in vitro studies in the presence and absence of the critical pro-survival cytokine IL-7 [26].
An adaptor protein, Grb2, one of the key proteins in the MAPK signaling cascade, is also an important co-mediator protein for the PI3K-Akt pathway which affects cell survival pathways.
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It has been reported that extracellular matrix (ECM) affects cells survival, proliferation, and migration [ 32].
Cryopreservation did not affect cell survival.
In addition, this peptide did not affect cell survival measured with the MTT assay.
Mechanical stress is widely known to affect cell survival and extracellular matrix metabolism in many cell types.
Labelling did not affect cell survival of mononuclear cells as measured by the MTS cell survival assay (n = 12, p > 0.05; Fig. 4b).
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