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We first examined whether mRNA nanocarriers can perform specific transfection of CD34+ human HSCs without affecting viability or triggering differentiation.
The activity resided on the surface-exposed membrane protein fraction of the mycoplasma, since mild trypsin treatment not affecting viability was shown to reduce suppressive activity.
Further, AA6 reduced serum-stimulated chemo-attraction (Suppl. Figure 3c; Suppl. Figure 4c) and adhesion to TNF-α-activated endothelium (Suppl. Figure 3d; Suppl. Figure 4d) without affecting viability (Suppl. Figure 3e; Suppl. Figure 4e), survival and proliferation (Suppl. Figure 3f; Suppl. Figure 4f).
Buthionine sulfoximine (BSO), an inhibitor of GSH synthesis, markedly reduced T cell proliferation without affecting viability and blocked production of IL-2 and IL-6 [31].
We first demonstrate that T cells can be efficiently loaded with 45 nm gold colloid nanoparticles without affecting viability or function (e.g. migration and cytokine production).
They found that 50 nM of Ag-NPs significantly arrested biofilm formation without affecting viability, whereas 100 nM inhibited the growth of the P. aeruginosa itself and led to 95%reductionon in biofilm.
Similar(39)
Agonists of REV-ERBs do not affect viability of normal proliferating and quiescent OIS cells.
Importantly, NP-transfection did not affect viability or proliferative activity of expanded HSCs (Fig. 6c, d).
This contamination negatively affects viability of sperm after cryopreservation.
Global deletion of Syndecan2 in mice does not affect viability and fertility of mice.
Fotaki, V. et al. Dyrk1A haploinsufficiency affects viability and causes developmental delay and abnormal brain morphology in mice.
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