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Unlike contemporary physical methods that affect all tissue components, PEF is an intervention at the cellular level, which precisely targets cell membranes through electroporation without affecting the extracellular matrix architecture14.
As these changes in mice have been shown to resemble those in human diseases, mouse models are likely to provide efficient tools for pathogenetic studies on human disorders affecting the extracellular matrix.
Similarly, deleting mutations (exons 8 and 9 of CDH1) affecting the extracellular cadherin-binding domains of E-cadherin show increased EGFR activation [30].
Conversely, the responses of gingival fibroblasts to mechanical loading are almost wholly unknown, which is perhaps surprising since orthodontic forces are constantly affecting the extracellular matrix (ECM) and the cells within dental pulp, periodontal ligament, alveolar bone, and gingiva [7] [9].
Two common co-segregating polymorphisms affecting the extracellular domain of the TLR4 (and299Gly and Thr399Ile) have been described in humans.
The ultimate goal of decellularization protocols is the removal of all cellular components without affecting the extracellular composition, mechanical integrity, and biological activity.
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The PAMNPs did not affect the extracellular content of TNF- α (B) and IL-1β (C) in the THP-1 cell line.
Bupropion did not significantly affect the extracellular 5-HT concentrations in all the 3 brain areas tested.
However, it is critical to ensure that both the SG processing and cryopreservation of these allografts do not detrimentally affect the extracellular matrix architecture within the tissue.
The concentrations for α-Phellandrene at their MIC (1.7 mL/L) and their MFC (1.8 mL/L) significantly affected the extracellular conductivity of P. cyclopium cells.
Although α-terpineol treatment did not affected the extracellular conductivity at initial exposure time, α-terpineol caused a significant increase after 30 min incubation (Fig. 2b).
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