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The greater peroxidase activity and lignin content are likely to be responsible for changes affecting cell wall stiffness and expansion of fruits yielded by TAGL1 silencing plants.
These results together with the higher sensitivity of septin mutants to drugs affecting cell wall strongly support the idea that in U. maydis septins are required for an efficient cell wall construction.
It is noteworthy that both TOR and Hsf1 function have been previously implicated as being involved in aspects of cell wall integrity via effects on the PKC/Mpk1 cascade [54], [74], [75], and deletion of genes affecting cell wall integrity can affect rapamycin resistance, and potentially TOR [16].
Some were defective in cell size and demonstrated misregulation of genes predominantly affecting cell wall development.
However, certain mutations affecting cell wall stability increase chitin levels to as much as 20% of total wall polymers [ 73].
The claim that pavement cell shape relies on oriented MTs affecting cell wall anisotropy has not actually been clearly demonstrated.
Similar(42)
In S. cerevisiae, deletion of GAS1 also affects cell wall composition [21].
Taken together, our findings indicate that a mutation of the SSN8 gene affects cell wall structure and integrity in C. neoformans.
It was proposed that a balance between these two proteases affects cell wall hydrolysis and lysis of a subpopulation of cells resulting in release of genomic DNA and enhanced biofilm formation [12].
Therefore, we tested the sensitivity of septin mutant cells at permissive temperature (22°C) to compounds described to affect cell wall integrity in this fungus (i. e. caffeine, Calcofluor White and chlorpromazine) [31].
The mutation did not significantly affect cell wall localization of the protein (Figure 1), secondary structure (Figure S2), or binding of soluble Als5p to polystyrene or fibronectin (Figure S1).
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