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Groove widths significantly influenced cellular alignment, with 50 μm grooved patterns affecting alignment most substantially.
On the other hand, it is also possible that stringent assembly procedures and shorter reads affecting alignment stringency have contributed to increase the number of unaligned reads.
To understand the factors affecting alignment extension with phylogenetic and profile-based methods, we tested PAGAN and hmmalign with idealized data mimicking an RNA-seq study (see Section 2 Fig. 3b, c).
On the other hand, it is also possible that stringent assembly procedures and shorter reads affecting alignment stringency and insufficient overlap have contributed to an increase in the number of unaligned reads.
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This does not just affect alignment in the obvious yoga poses, like seated or standing forward bends.
We therefore speculate that either: (i) hippuristanol interferes with proper interdomain interaction, which in turn abolishes RNA binding or (ii) affects alignment of Thr329 with its target phosphate on RNA.
For example, how do reads with various sequencing qualities affect alignment results?
Existing methods determine this parameter more-less arbitrarily, which could affect alignment quality.
Although SNP tolerance does not increase alignment yield substantially, it affects alignment results in 7 8% of transcriptional reads, typically by revealing alternate genomic mappings for a read.
Also, which amount of sequence information is used within NCF does not affect alignment quality, while the inclusion of topological information is crucial for producing good alignments.
This could affect alignment slightly, in the case of non-annotation guided alignment, for a small number of ambiguously aligning reads.
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CEO of Professional Science Editing for Scientists @ prosciediting.com