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A cytotoxicity test using the MTT assay showed that within solubility limits (<30 μM), none of the tested compounds significantly <span class="lh">affected viability of human fetal mesenchymal stem cells.
Furthermore, neither the p38 kinase inhibitor SB203580 nor the JNK inhibitor SP600125 affected viability (Figure 1B).
We did not find that deletion of FUN30 affected viability in these assays, as shown for growth under elevated temperature (Figure 2A, data not shown).
Cell viability was determined by trypan blue exclusion, and none of the RNAi targets used affected viability compared to the negative RNAi control or mock-treated cells.
SPARC deficiency only marginally affected viability.
Over-expression of AcGi, AcGq, or AcGs in the background of dopaminergic (THGAL4 )-driven dFz2 knockdown either did not rescue flight or affected viability.
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This contamination negatively affects viability of sperm after cryopreservation.
99mTc-HMPAO labelling did not affect viability of cryopreserved mononuclear cells as measured by the MTS cell survival assay (Fig. 4b).
Buthionine sulfoximine (BSO), an inhibitor of GSH synthesis, markedly reduced T cell proliferation without affecting viability and blocked production of IL-2 and IL-6 [31].
We first demonstrate that T cells can be efficiently loaded with 45 nm gold colloid nanoparticles without affecting viability or function (e.g. migration and cytokine production).
Bacterial aggregation can affect viability.
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