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This result implies that CC-EO and trans-cinnamaldehyde affected the affinity of the enzyme for L-DOPA but did not bind at the active site (Webb 1963).
Based on structure comparisons, we designed a set of talin F3 mutations that selectively affected the affinity of talin for its targets, as determined by stopped-flow fluorescence measurements.
The fact that a deficiency of SSI, BEI, or BEIIb affected the affinity of other starch biosynthetic isozymes for the starch granule implies that there is a close interaction among SSI, BEI and BEIIb during amylopectin biosynthesis in rice endosperm.
To determine whether the structural changes noted by CD affected the affinity of PPARα for LXRα, the protein protein binding experiments were repeated in the presence of LCFA and LCFA-CoA.
Both heparin and IP3 dimer completely displaced specifically bound [H]IP3 from NT1, but thimerosal (100 μM, 10 min) affected the affinity of neither: Δp Kd was 0.11±0.09 and −0.06±0.19 for heparin and IP3 dimer respectively.
However, if the Ser667Phe mutation simply affected the affinity of the mutant band 3 for GPA, then a titration of GPA with S667F-B3 would be expected to show a linear relationship.
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Conversely, binding of these substances to hemoglobin affects the affinity of hemoglobin for oxygen.
Furthermore, as reported above for IAA, the deprotonation of the carboxylic acid on 2-NAA and 2,4-D significantly affects the affinity of these molecules toward CB[7].
Thus, we can assume the morphology of the ZnO nanocrystals must be an important fact that affects the affinity of phenol compounds to HRP.
In our kinetic experiments, this deletion did not affect the affinity of protoporphyrinogen IX nor the FAD content, but decreased the catalytic efficiency of the enzyme.
A single point mutation at position 131 replace an arginine (R) with a histidine (H) that can affect the affinity of the receptor for human IgG subclasses.
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CEO of Professional Science Editing for Scientists @ prosciediting.com