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The analytic consequences of this preservative are of primary importance to investigators analyzing affected specimens.
The Dll Fta mutation and mildly affected specimens recovered from Dll RNAi in T. castaneum closely resemble weak hypomorphic combinations of Dll alleles in D. melanogaster (Panganiban 2000).
In moderately and severely affected specimens the distal tibia, near the tibia−tarsus joint, was also abnormally shaped, with absent or misplaced tibial spurs.
All visually affected specimens showed positive results for at least 1 organ and for at least 2 of the following tests: microscopy (Ziehl-Neelsen staining), in vitro cultivation (LJ medium and charcoal medium), IS 2404 nested PCR, and histopathologic examination.
Noteworthy, all ovarian tissue of the 11 visually affected specimens showed positive results for at least 2 tests.
Loss of the coxa-trochanter joint was also observed in severely affected specimens (3 of 16 scored).
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The cooling method does not affect the ultimate bond stress of the specimens using uncoated rebar very much but can greatly affect specimens that contained epoxy coated rebar.
The two most important parameters affecting specimen curvature is the overlap length and the thickness of the aluminium plate in the joints.
However, unless systematic differences were present by site in terms of the severity of illness affecting specimen collection, this variability should have been nondifferential and biased the findings toward the null.
In mildly affected RNAi specimens, the only defects we observed were in the tarsus, where t1 was deleted or fused to the distal tibia.
RT-PCR detects norovirus at lower concentrations and is less affected by specimen quality and preparation than electron microscopy [ 3- 5]; large numbers of specimens can be tested simultaneously, compared to the single throughput for electron microscopy.
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