We note that compounds such as camptothecin and psoralen, and even endogenous DNA lesions would affect transcript elongation and result in considerable transcription stress, likely resulting in a need for the transcription function of RECQL5 described here.
Recent reports have suggested that modulating transcription of Rv2745c or the M. smegmatis homologue affect transcript levels of the clp proteases [32], [33]; however, these studies did not assess whether or not this is a direct effect.
Increased expression of OsIDS1 did not affect transcript levels of the AP2 genes (Figure 3B and C).
Therefore, it appeared that alterations in LOV1 activity did not affect transcript levels of the circadian clock genes.
Interestingly, small variants were significantly more prone to affect transcript levels suggesting a preferential localization on (long distance) gene enhancers and repressors.
Central Europeans exhibit selection of specific ATXN2 CAG-repeat alleles that do not result in a different protein sequence, but may affect transcript or protein abundance (40).
However, many of the SNPs we identified are likely to affect transcript structure in vivo, and the synthetic association study approach can be easily generalized to any computed genome annotation to uncover relationships between genotype and important molecular phenotypes.
A stable target-probe heteroduplex produces a detectable signal, whose interpretation depends on the experimental details, such as how target processing steps affect transcript abundance in gene expression experiments [10], [11], and whether probe sequences match sample SNP alleles [12].
