We investigated whether the osu1 alleles affect the transcript levels of two MYB transcription factor genes, MYB75/PAP1 and MYB90/PAP2, which are involved in activating anthocyanin biosynthesis in response to C and N nutrient status [39], [43] [46].
We performed real-time reverse transcription (RT PCR to determine whether treatment with benzyl-α-GalNAc would affect the transcript levels of MUC1 mRNA.
This indicated that although Glu and CNQX regulate the OsGLR mediated [Ca2+]i increase in rice roots, they do not affect the transcript level of OsGLRs.
Similar to UCP1 expression, this treatment did not affect the transcript levels of PPARγ or GPDH, two markers of adipocyte differentiation.
As shown in Figure 4B, treatment of myotubes with TWEAK for 18h did not affect the transcript levels of Dicer, Dorsha, or Exportin-5 indicating that TWEAK may be affecting the expression of miRs but not their processing from pre-miRs to mature miRs.
In summary, SA did not significantly affect the transcript abundance of any of the BnWRKYs tested, whereas ET, ABA, JA and the cytokinin BAP did affect the transcript abundance of various BnWRKY genes investigated in this study (Table 2).
Berulava et al. showed that altered FTO levels affect the transcript of genes related to RNA processing and metabolism [ 3].
For example, genetic variation in upstream splicing factors of the sex hierarchy are known to affect the transcript abundance for downstream targets like Yolk protein [ 26].
In low glucose media, that is, with activated AMPK, the specific AMPK inhibitor Compound C did not affect the transcript levels of IRF1 and APOC3 (not shown).
However, agroinfiltration did not significantly affect the transcript levels of endogenous N. benthamiana homologous genes in GFP-infiltrated plants (Fig. 9a).
