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Whether this spikiness would affect the sequencing results was not known at this stage.
PCR duplication reads during sequencing, which affect the sequencing depth and variation detection, were excluded by an in-house script.
A high GC content region can affect the sequencing depth for exome sequencing, which makes identifying CNVs using exome sequencing data even more complicated.
It was not known if this would affect the sequencing library quality, so one sample was split and bead clean-up carried out on one of the sub-samples, to compare results with its uncleaned counterpart and assess whether this discolouration had any effect on the sequencing results.
Similar(56)
However, heating rate as an important processing parameter does not affect the sequence of these reactions.
For example, a genetic code based on quadruplets could be artificially designed so that a single substitution of a single nucleotide in nearly any position of the template would not affect the sequence of the encoded protein.
In addition to influencing the pathway organization, transposition and recombination of IS6100 elements can affect the sequence of Lin enzymes.
The authors concluded that "taste receptors seem to be able to partially affect the sequence of events during swallowing".
This suggests that the globin transcripts in the sickle cell sample do not affect the sequence reads and do not introduce much bias in the analysis.
These studies show that loops 1 and 7, especially loop-7 (previously named the 'specificity' loop), affect the sequence motif specificity [ 58– 66].
Importantly, PV and none of the described human mutations affect the sequence of the TRα2 isoform that is also expressed from the THRA locus but which cannot bind T3 and has no known physiological function.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com