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While the use of CPAs such as dimethyl sulfoxide (DMSO) and glycerol mitigate this damage, their toxic nature necessitates removal after thawing and this can adversely affect the functionality and integrity of the recovered cells, tissues or material.
For one, a tag may affect the functionality and proper localization of proteins.
Specifically, the underlying assumption is that the (5 base pair intronic) deletion in the Alpha-2-macroglobulin gene may affect the functionality and quantity of the resultant Alpha-2-macroglobulin protein in circulation, therefore contributing to Alzheimer's disease pathology [22].
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The architecture of the thylakoids is a major factor affecting the functionality and efficiency of the photosynthetic apparatus.
Milk components, in particular fat and protein, affect the functionality, yield and quality of dairy products, requiring a clear understanding of the structural and chemical changes occurring due to PEF processing.
HP can affect the functionality of protein and carbohydrate molecules often in unique ways, which may allow optimization of food manufacturing processes and production of novel foods.
Number of substitutions common to the various human phenotypes of AR diseases and AR-A and AR-B in fish (see Additional file 6 for details) The above analyses suggest that these sequence differences between AR-A and AR-B will affect the functionality of these receptors and are linked to a putative neofunctionalization event.
The isolation of monocytes and their tissue culture plates might affect the functionality of the cells and modify them relative to their in vivo activation state.
In blood leucocytes, cytokines and mediators may affect the functionality of extra-pulmonary tissues and organs, leading to COPD-associated comorbid conditions.
We envision that multiple factors could affect the functionality of viral siRNAs and therefore limit their regulatory potential on host targets in vivo.
It is conceivable that these alterations affect the functionality of both stem and progenitor cells.
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