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In summary, kinetic and thermodynamic calculations indicate that deglycosylation does not affect the affinity of binding, but a heterologous associated C region will, as shown above.
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Instead, the DNA binding domain might affect the affinity of DNA binding and consequently the directionality and efficiency and/or processivity of nucleosome repositioning.
A second possibility is that differences in the post-translational modifications of tubulin in the intra-conoid MTs and the cortical MTs may affect the affinity of TgICMAP1 binding.
The protonation state of these is likely to affect the affinity of the binding site for Na+.
We hypothesize that the -271 G>A polymorphism is contained in a response element of the gene and that the different KDR genotypes may affect the affinity of DNA binding protein for the gene promoter, resulting in different transcriptional activities.
There exists a theoretical possibility, therefore, that membrane-bound blood group substances on CD4-positive cells may thus affect the affinity of viral-binding proteins and viral infectivity, promoting or diminishing cell susceptibility to infection.
It remains to be determined if the residues implicated in this study affect the affinity of PA-ANTXR2 binding at neutral pH; the long half-life of the complex (15 hours) [19] precluded the use of moderate throughput analysis of all the mutants.
These structural modifications affect the affinity of the TTR-binding site not only to T4 but also to exogenous compounds, and explain the differences in the TTR-binding potency of compounds reported between species.
Cytosine methylation may directly affect the affinity of transcription factors (TFs) towards their binding sites (TFBSs) [ 45].
If it is a cause, then DNA methylation may affect the affinity of transcription factors (TFs) for their binding sites (TFBSs).
To address whether H3K64ac affects the affinity of histone DNA binding, we performed competitive nucleosome reconstitution assays (Thåström et al., 2004).
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