Taking advantage of expression data from mouse Pax6Sey/Sey embryonic telencephalon [17], we next compared the 559 lens gene list (see above, Supporting information) with differentially expressed genes in E12 and E15 dorsal (cortex, Ctx), and E12 and E15 ventral (the ganglionic eminence, GE) telencephalon [17] as described in Methods.
One advantage of expression profiling methods for characterizing microglia is that they permit studies of genes not readily amenable to antibody staining (e.g., soluble chemokines/cytokines).
Another key advantage of expression profiling studies is that they also inform about the consequences of epigenomic modifications, as transcriptomes reflect not merely the output of DNA sequences, but also their interplay with non-genetic modifiers of gene expression.
The advantage of expression analysis by Western blot, compared to dot-blots, is that this allows one to quantitate the expression levels of full-length constructs and eliminate the contribution from cleaved protein tag.
We took advantage of expression profiling of ovarian and endometrial cancer cell lines treated with demethylating agents to identify candidates for epigenetically silenced tumor suppressor genes in epithelial ovarian cancer.
It should be kept in mind that spermatogenesis is much more efficient in mouse compared to man, yielding a much higher number of mature spermatozoa per gram testis weight per time unit, and a small selective advantage of expression of an X-linked gene in mouse spermatids may translate into an essential function in man.
Antibody engineering can also be used to generate single-chain variable antibody fragments (scFvs), which are composed of the variable regions of heavy and light immunoglobulin chains joined by a flexible linker; these offer the advantage of expression as a single molecule (Fig. 3).
Plant-based expression systems combine the advantages of eukaryotic expression with a up-scaling potential and a good product safety profile suitable for vaccine production.
Taking advantage of expression-profiling surveys performed in extended human populations [ 171- 174], systems biologists have started integrating physiopathology, network biology and DNA variations [ 175- 177], providing novel insights into the mechanisms of various diseases, such as diabetes [ 178] and obesity [ 179].
Production of recombinant antibody fragments (Fabs) in Escherichia coli has gained interest because of the recognised advantages of this expression system and because Fabs do not require glycosylation.
