The Roche/454 FLX system is advantageous when longer reads are important (average read length 700 bp), whereas the Illumina system provides deeper sequencing coverage at a reduced cost per base, albeit with shorter read length (average length 100 bp).
It's an outlet, a mutually advantageous show.
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Though Sanger's longer reads were advantageous in extending an assembly, the generation of 18,424 Sanger reads was associated with much higher cost.
In contrast, the 454 pyrosequencing platform, which generates fewer but longer reads, is advantageous in shotgun metagenomics as these longer reads enable better functional assignments of sequenced gene fragments.
Furthermore, prior information can be taken into account in a direct way within a single optimization problem, which we think will turn out particularly advantageous for integrating long reads from third-generation sequencing platforms with RNA-Seq data.
Third, the inclusion of a non-numerical control condition would be advantageous to exclude influences on brain activation that cannot be attributed to cognitive styles (e.g., reading speed, meta-cognition).
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In general, Roche's sequencing technology produces long reads and is advantageous for assembly of sequences into longer contigs; however, the number of reads generated in each run is lower than that of other platforms and not enough to reach deep coverage for low-abundance genes.
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This is especially advantageous in comparing numbers of cDNA-originated FLX reads to gain insights into gene expression.
