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N6-Naphthalenemethyl-2′-methoxybenzamido-β-NAD+, a derivative of a low micromolar first-generation inhibitor of trypanosomal glyceraldehyde phosphate dehydrogenase (GAPDH), was synthesized, taking advantage of methodology for the selective phosphitylation of nucleosides.
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Taking advantage of methodologies that are highly amenable in the zebrafish system, we conducted a targeted investigation of molecules involved in ECM deposition, remodeling and turnover in wild-type (WT) and ML-II embryos.
Only 5 μg of protein were necessary to perform the whole procedure, since we took advantage of methodologies that require minimal amounts of protein: i) in vitro protein expression and purification, ii) antibody phage display and iii) scFv antibody microarray screening.
A combined simulation analytical methodology takes advantage of both methodologies' techniques and benefits, and the process can be repeated until an acceptable set of outputs and alternatives is found (Fig. 6).
Another advantage of this methodology is the reduction in uncertainties.
Major advantage of this methodology is to avoid toxic reagent and intermediate.
The numerical results we obtained justify the advantage of this methodology.
An unquestioned advantage of this methodology will be possibility to evaluate if isolation exists or not.
Two examples are given to illustrate the validity and advantage of provided methodology.
A comparison with transit time ultrasonic flow meter shows the advantage of proposed methodology.
The advantage of the methodology is that each subsystem can be designed independently of others.
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