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Advanced populations, such as recombinant inbred lines, have been broadly adopted for QTL mapping; however, construction of large advanced generation crop populations is time-consuming and costly.
Advanced populations such as recombinant inbred lines (RILs) and nearly isogenic lines (NILs) are frequently used for QTL mapping to reduce the cost of genotyping due to the high frequency of recombination within a limited population size [ 10– 10].
However, there might be extra loci or genetic factors playing a role that could be unravelled if the population size is increased or when advanced populations are further explored.
The identification of the additional locus/loci will require significant population development and advancement, as detection of deletions is most successful with advanced populations which have low heterozygosity.
We hope to obtain some key recombinants in the qRgls2 locus when screening more advanced populations.
Breeding programs are usually reluctant to evaluate and use germplasm accessions other than the elite materials belonging to their advanced populations.
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"Evolutionary biology is not in good shape in the Netherlands," says Galis, noting that the dismissals will wipe out Leiden's expertise in theoretical biology and advanced population modeling.
Controlling the false positives and negatives for the highly structured, advanced population studied here would always be a challenge, especially when there is selection in favor of some other agronomic traits.
During postglacial expansions the advancing populations may encounter a variety of unoccupied habitats with varying selection regimes, which could drive diversification.
During rapid postglacial range expansions advancing populations encounter a wide variety of unoccupied habitats with varying selection regimes, which could drive diversification in comparatively short periods of time.
Over time, ethanol concentration in the feed was raised as yeast tolerance improved and advancing populations were captured in glycerol stocks for use as described above.
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