However, considering more advanced PCR-based tests for unknown or unrecognized adventitious agents (e.g., representational difference analysis, use of various degenerate primers) would require extensive research and add substantially to overall costs.
17 18 20 40 41 In AERIS, samples will be acquired during AECOPD and stable disease and analysed for a wide range of potentially pathogenic bacteria and viruses using advanced PCR-based techniques as well as traditional culture-based methods.
To investigate whether CYP2C19 activity was decreased in patients with advanced cancer, CYP2C19 genotype was determined in 33 advanced cancer patients using PCR-RFLP analysis for the two important allelic variants (*2,681G>A and *3,636G>A) and the activity of the enzyme was evaluated using the CYP2C19 probe drug OMP.
Definitive diagnosis of HIV infection is only possible using advanced technical approaches such as PCR to confirm presence of the virus in the blood [3].
Real-time polymerase chain reaction (PCR) technique has advanced greatly over the past 10 years.
Recent advances in PCR chemistry [47], [48], [49] mediated significant improvements in the robustness of autosomal STR typing assays by overcoming some of the adverse effects of PCR inhibitors.
Advances in PCR based genomic approaches have generated robust DNA marker systems [ 3, 4], which offer an effective approach to augment breeding methods for mulberry improvement [ 5].
Advances in PCR technology using the real-time fluorescent monitoring capabilities of the LightCycler have the potential to enable relatively inexpensive, rapid and accurate quantification of gene amplification [ 24].
The most recent advances in PCR methods are focused on the reduction of the limits of detection and quantification [ 27, 28], on the discrimination between dead and live cells [ 22] or the simultaneous detection of different foodborne pathogens [ 22, 27– 29].
In this study, we have evaluated an 82 angiogenesis-related gene set in a series of 61 advanced ovarian carcinoma samples by quantitative real-time PCR (qRT-PCR).
