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In advanced passages the cells ceased expression of nestin, contained GR in the nucleus, and concomitantly halted proliferation.
Strikingly, KGN cells at both early and advanced passages metastasized to the bowel when injected s.c. into nude mice.
These cells maintained a high proliferation rate even at advanced passages without modification of morphological aspect (Fig. 3d).
Interestingly, hMSCs presented a higher coefficient of variation in G0/G1 and G2/M peaks at advanced passages in culture, suggesting the presence of abnormal cells with a DNA content not equal to 2N or 4N (aneuploid); hTERT-MSCs maintained coefficients similar to hMSCs at early passages.
More interestingly, KGN cells at both the early and advanced passages metastasized from the subcutaneous transplanted region to the bowel, especially in the submucosa of the small intestine, but not to the other tissues (metastases in the bowel were seen in 7 out of 9 mice transplanted) (Fig 4B).
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For detailed characterisation of KGN cells, early and advanced passage cells were cultured, and the proliferation capacity was monitored.
The chromosomes were examined in exponentially growing early and advanced passage KGN cells (early: P6; advanced: P53) in an in vitro culture.
In contrast to frozen digests, in flow cytometry of cultured cells, this background excludes the use of multicolor staining in order to avoid severe compensation problems, at least when using advanced passage numbers.
In advance passages of her speech released by aides, the home secretary will say: "I know there are those who say that our reforms have gone too far, that the pendulum has swung too much the other way, and that reforms to stop and search are linked to knife crime in our capital and elsewhere.
As shown in Fig 4A, the xenograft of the advance passage cells developed faster than that of the early passage cells at the region where KGN cells were injected, and larger clumps under the skin were observed (in the small windows of Fig 4A).
Selma (Rev. Dr. Martin Luther King, Jr.'s work in leading the 1965 Selma March and advancing the passage of the Voting Rights Act).
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