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Since patients with a low abundance of EGFR mutations may achieve more clinical benefits from EGFR-TKI treatmore, more sensitive and advanced assays, such as droplet-digital PCR (next-generation quantitative PCR) or next-generation sequencing [ 46, 47], may be a preferable option to more accurately evaluate EGFR mutation status and confirm "true" wild-type EGFR NSCLC.
These advanced assays could be applied to screen easily and rapidly a broad spectrum of molecules inhibiting cancer cell endothelial adhesion and extravasation, thus contributing to the design of more focused in vivo tests.
This approach holds great promise for powering portable diagnostics, as the generated electric power could enable device functionalities required for advanced assays, such as device timing, actuation, and signal quantification.
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Notions of compound quality have progressed and should be informed by a deep understanding of the complex intersection of chemical space and biological target space, which may be termed bioactivity space, and in the context of advancing assay methodologies.
However, since we planned to evaluate the vaccine-induced immune responses using a virus challenge advanced immune assays were not necessary.
HPO performed advanced PCR assays that contributed to a better understanding of the effects of primer dimers and non-specific PCR extension products on the HTS-PTT assay for BRCA analysis and contributed significantly to the drafting of the manuscript.
The emergence of near complete genome sequences, next-generation sequencing (NGS) technologies and advanced functional assays in plants, has pushed forward those boundaries by allowing systems level investigations of traits and processes that were unveiled by genetic and biochemical studies.
We tend to favor the second model, as we often see no evidence of an advancing furrow (assayed using dac expression as a marker).
Precision Red advanced protein assay (Reagent #2) was from Cytoskeleton (Denver, CO, USA).
The total protein concentration in each lysate was determined by the Precision Red advanced protein assay reagent in the kits.
In addition, recent advances in assays and collection techniques have made assessment of insulin secretion using C-peptide less expensive, more reliable and widely available.
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