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Top2 -complementing males were considered fertile if adults were produced from the cross.
At 0.1 μ m of the drug, a substantial number of tub-AOX expressors even reached eclosion, whereas only a few viable adults were produced at this dose by control flies under Surf1 knockdown alone (Fig. 2C).
Over-expression of wild-type p53 at high levels during larval development was toxic to both males and females, in that no male adults were produced, and only three female adults (escapers) were obtained.
No more than five notonectids were added into any cage even if more than ten were collected because earlier experiments indicated that no adults were produced in cages with higher numbers of notonectids.
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Approximately 90% of systemic EPO in adults is produced by peritubular interstitial fibroblasts in the renal cortex and outer medulla of the kidney.
Incubation was performed as described above until F1 young adults are produced.
Near the end of the summer, the next generation of reproductive adults is produced and the colony senesces.
New adults are produced every week by allowing approximately 300 eggs to be laid in a new bottle added to each cage.
The efficiency of gene inactivation in touch receptor neurons was tested by incubating strain ID447 larvaevae in 96-well plates with bacteria expressing either RNAi against GFP (plasmid L4417) or empty vector (plasmid L4440) until F1 young adults are produced.
We therefore investigated whether IL-10 in adult was produced in larger amounts in the intestine or MLN, potentially accounting for lower IL-12 response observed to R-848 stimulation.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com