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Results showed that, by adopting overlap PCR strategy, the gfp gene was successfully inserted into and fused with PRRSV Nsp2 gene in the PRRSV infectious clone plasmid FL-12 to generate FL12-GFP plasmid.
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Rather, it is in all three cases defined based on the sole primitive notion adopted: overlap in SA, and discreteness in the other two systems.
This leakage is filtered through passband filtering, resulting in phase errors at the edges of the frame.In order to remove the phase errors, we adopt overlapping processing of the FFT frame.
A sequential thermo cycling program was adopted for overlap extension reaction and amplification of spliced DNA.
Mirroring the latest evidence on the relationship between frailty syndrome and depression, it was found that, even in the cross-sectional investigation performed in the present study, that the approach adopted identified overlapping populations, i.e. that exhibited both syndromes.
In prediction of protein complexes by DPClus, we adopted the "overlapping clustering mode," which allows identical proteins to be classified into different clusters, because it is biologically well established that proteins can be present in multiple complexes at different times and locations.
We adopted the overlapping method (8-aa overlap) to synthesize a total of 191 nonapeptides (9-mers) covering HBc1 150 aa (Zhang et al., 2013).
This is well-known to be true for self-avoiding walks and ring polymers, where the effective potential at full overlap
The WD-repeats of RACK1, as with all proteins that adopt this fold, overlap two adjacent propeller blades to provide an interlocking architecture.
This approach was adopted to avoid overlap with dimensional irritability/aggression, which is present across different child and adolescent mental disorders (e.g., ADHD, CD, ODD, MDD, PTSD, and disruptive mood dysregulation disorder).
In this paper, we analyze length, polarity and phase properties of genetic overlap in prokaryotes adopting a common framework of genome compression [ 8].
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