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Of the various procedures we adopted, that using a mixture of 0.2 % cellulase, 0.2 % cytohelicase, 0.2 % pectolyase and 0.05 % glucanase for a hydrolysis time of 4 h gave PMCs with no callose and clear cytoplasm, and after FISH showed ∼80%% of PMCs with chromosomes containing hybridization at NORs with no background, and allowed a clear visualization of hybridization signals (Table 2).
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Detailed food consumption data are not available for all European countries and so a hierarchical approach was adopted that used simple food balance sheet (FBS) data for all European countries and summarised individual data for countries represented in the European Food Safety Authority database.
To increase assay sensitivity, a heminested PCR format was adopted that used a sense primer designed in a segment of the untranslated region that is highly conserved among all anelloviruses (5´-TCAAGGGGC AATTCGGGCT-3´).
A content-based method is adopted that uses musical content as the basis for the SOM clustering.
A new approach to the problem is adopted that uses impedance and mobility methods commonly found in the analysis of purely structural or purely acoustic systems.
To remove a fault faster, an approximation technique is adopted that uses the information from ½ or ¼ of the period to represent the whole period to reduce the operational zone of the protection but still guarantee the selectivity.
In this paper, an improved ultrasonically assisted z-pinning (UAZ) process was adopted that uses an acrylic board with holes as a mould to hold the pins instead of a polymer foam carrier.
There is currently no standardized method for doing this so we propose that an automated and verifiable method be adopted that can be used to compare international studies on diagnosis and pathogenesis.
First, it developed or adopted components that use less power (Bluetooth 4.0, for example).
To exemplify the possible use of such wind schedules in the design of control laws, model-based controllers are formulated in this paper that are capable of performing power curve tracking throughout all wind speeds, in contrast with commonly adopted approaches that use switching controllers to cover the various operating regimes of the machine.
Activity counts were calculated with an algorithm adopted from that used by the Actical Physical Activity Monitor Philips Respironicss, Bend, Oregon).
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