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Switching the relative positioning of these residues in SeCobAW93F/F91W likely causes the Co II)rrinoid substrates to adopt a different orientation within the enzyme active site, especially at the low temperatures used in our spectroscopic experiments.
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The NTD of eArgRS adopted a different orientation from that of yArgRS as well.
Indeed, the phenol ring, which adopts a different orientation in BPC, induces a 180° rotation of T347 (H3) and forms a hydrogen bond with the hydroxyl group of this residue.
In the crystal structure, however, the ligand appears to bind in its neutral form and adopts a different orientation, making only one hydrogen bond with Asn152 while the aryl oxygens hydrogen bond with structural water molecules (rmsd 3.5 Å).
Since this helix must adopt a different conformation or orientation within the closed dimeric state, it might be the element that locks TAp63α in its tetrameric form after phosphorylation induced activation.
Will the Brown government adopt a different approach?
"It was not possible to adopt a different attitude.
So we had to adopt a different mindset.
However, here we adopt a different approach.
The co-crystal structure of Ac-AChBP with a more potent analog TxIA(A10L), revealed that this α-conotoxin adopts a different binding orientation to that observed for other α-conotoxin AChBP complexes.
This purine base binding plane is sandwiched between the side-chains of Ile66 (Val66 in hsCK2α) and Met163, and it adopts a significantly different orientation than in prominent homologues like cAMP-dependent protein kinase (CAPK).
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