The phrase "ado reducing" is not correct and does not make sense in written English.
It seems to be an attempt to convey a concept related to reducing something, but it lacks clarity and proper structure.
Example: "The process of ado reducing is not well understood in this context."
Alternatives: "minimizing fuss" or "cutting down on delays".
In this paper, ADO and the endogenous reducing system including Fd and FNR from Synechococcus elongatus PCC7942 were cloned, overexpressed, purified, and characterized.
In contrast, the levels of Ado and R2-Ado were significantly reduced (Fig. 1d), suggesting that the branched PAR chain polymerization is notably affected in the absence of PARP2.
The use of optimized cell lysis and DNA amplification conditions, along with sensitive detection systems, should reduce ADO to a minimum.
The results of the hybrid Fd/FNR reducing systems demonstrated that ADO was selective against Fd.
(3) The hybrid experiments of the surrogate and the cognate biological reducing systems demonstrated that ADO is selective against Fd and the interaction between Fd and FNR is very important for efficient electron transfer and ADO activity.
For the first time, ADO was functionally in vitro reconstituted with the endogenous reducing system from cyanobacteria, which supported higher ADO activity than the surrogate Fd/FNR system and the chemical one, and produced less H2O2 than the heterologous one.
The reactions contained 2 mM n-heptanal in 100 mM HEPES buffer (pH 7.2) containing 100 mM KCl, 10% glycerol and 4% DMSO, 5 μM ADO, 20 μM ferrous ammonium sulphate, 10.26 μM Fd and 1.8 μM FNR from PCC7942, 2 mM NADPH (for the cognate reducing system), or 15 μM ADO, 60 μM ferrous ammonium sulphate, 75 μM PMS, 750 μM NADH (for the chemical reducing system).
We reported the first cognate reducing system from cyanobacteria to support ADO activity.
For the first time, ADO was functionally in vitro reconstituted with the endogenous reducing system from cyanobacteria, which supported greater activity than the surrogate and chemical ones, and produced less H2O2 than the heterologous one.
As mentioned above, the biological or chemical reducing system is required for catalytic activity of ADO [ 8, 14- 16].
Just like other ADOs, ADO 1593 was also active against these two substrates in the presence of the chemical reducing system (PMS and NADH) (data not shown).
