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Co-localization was found in septic patients, mainly in the admission samples, and not in healthy volunteers, indicating that increased NADPH-oxidase activity is a source of ROS in septic patients.
In the follow-up samples, decreased production of ROS and increased production of inflammatory cytokines were observed under all stimuli compared to the admission samples, which indicated a trend toward the restoration of homeostasis.
Admission samples were collected before starting therapy.
If patients had AKI on admission, samples were collected immediately after admission.
NPA samples for RT-PCR were taken from all patients at admission; samples from 156 patients (71.6%) were positive.
At admission, samples were available for analysis from 76 of the 81 patients (94 %) initially included (Fig. 1).
Similar(45)
In one of the patients there was a rapid decrease in concentration soon after the admission sample which might represent a distribution phase; the reason for this sample differing from the others is not apparent from this data.
An admission sample was obtained for 6047 patients (73%).
Importantly, a low IgG response in the admission sample was significantly associated with disease severity.
Comparison of the admission sample and the second sample taken 21 days after admission: both CD4 and CD8 were most decreased at admission, immunoregulatory index had a shift to positive values in the admission sample.
The six-year follow-up questionnaire was mailed to the surviving individuals of the index admission sample (n = 254).
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