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Over the last 20 years, cardiomyoplasty, based on the isolated administration of cells with regenerative capacity, has been the focal point of most studies aimed at regenerating the heart.
After administration of cells to infarcted hearts, they demonstrated increased vasculogenesis and myogenesis as well as more effective overall reparative activity [63].
Individual body weights were determined at randomization/animal receipt procedure, shortly before each administration of cells or vehicle control (dosing day), and thereafter twice weekly, and prior to the respective scheduled termination.
The process of harvesting human cells from patients with acute myocardial infarction, analysis of cell function, radiolabelling of monocyte subfractions, administration of cells into mice and finally performing imaging with either planar and tomographic (single-photon emission computed tomography (SPECT)) scintigraphy or positron emission tomography (PET) is a long process.
On day 7 after administration of cells animals were euthanized, perfused and tumors along with surrounding tissues were collected for histochemical analysis (Prussian blue staining).
Animals were intravenously administered unlabeled CTLs, magnetically labeled control T-cells or magnetically labeled CTLs on day 14 post-tumor implantation and MR images were obtained on days 3 and 7 after the administration of cells.
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With the rapid increase in approaches to pro- or anti-angiogenic therapy, new and effective methodologies for administration of cell-bound growth factors will be required.
Since numerous body locations have been explored as valuable options for the administration of cell microcapsules, this review offers a current vision of the possibilities this technology offers attending to the administration routes.
While the clinical administration of cell-based therapeutics often requires additional safeguarding and targeted delivery compared with traditional therapeutics, there is significant opportunity for minimally invasive device-assisted cell therapy to provide the physician with new regenerative options at the point of care.
a Plasma concentrations of free heme 3 24 h post i.p. administration of cell-free human Hb.
Intraperitoneal administration of cell-free Hb could be performed safely without local short- or long-term negative effects related to i.p. deposition.
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