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H-LW participated in design of the study, carried out animal drug administration, cell culture, immunohistochemistry, RT-PCR, western blot and Elisa analysis, and drafted the manuscript.
This together with the tunable properties of the gel system demonstrated here therefore indicates potential other uses, like within cell and biofactor administration, cell cultivation, tissue bulking and tissue engineering.
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Prior to administration, cells are thawed, washed and reconstituted at the clinical site, as detailed below.
To track the distribution of MSCs after systemic administration, cell-linker PKH26 (Sigma) was used according to manufacturer's protocol to label MSCs prior to injection.
After 8-day GST or GST-Nogo-66 administration, cells were fixed by 4% formaldehydum polymerisatum (Merck) for immunocytochemistry.
Once Cre-mediated recombination is induced upon tamoxifen (TM) administration, cells are genetically marked, therefore labelling does not dilute with cell division and growth.
In sequential administration, cells were initially exposed to freshly aggregated Aβ alone, followed by replacement of the Aβ solution with curcumin for 24 hrs patch clamp recordings were performed 24 hrs after curcumin treatment.
After DETA NONOate administration, cells were incubated for 4, 6, 8, 12, 16, and 24 h.
After STZ administration, cells that were not affected by STZ-induced necrosis showed increased mitotic characteristics.
After ALA administration, cells generate PpIX through the haem biosynthetic pathway.
The day after virus administration, cells changed their shape from an oval structure to a more flattened fibroblast-like shape.
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