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In those operative conditions, differently from common on-ground experimental activities, a non-specialist operator, an astronaut of the space station crew, is expected to self-administer the experimental protocol, particularly self-marking specific anatomical landmarks.
Artesunate was administered per the experimental protocol.
After the baseline study, dantrolene (10 μM) was administered for 20 minutes, and the experimental protocol was repeated.
Fluorescein isothiocyanate was bound to bovine serum albumin [ 17] and administered into the tail vein at 20 hours of the experimental protocol (66 kDa FITC-BSA, 200 μl/100 g intravenously; Sigma; Gillingham, Dorset; UK) to allow observation of the microcirculation.
We identified the trigger and end-points for fluid bolus administration, the number of boluses administered and the use of red cell transfusions and vasoactive medication as part of the experimental protocol.
The DNA STdR level at 2 h was somewhat higher following intravesical administration, however, the experimental protocol required that UVA irradiation was performed 20 h after STdR was administered.
The experimental protocol was approved by the University of Pennsylvania Institutional Review Board.
The experimental protocol is as illustrated in Figure 1.
The experimental protocol was approved by the Animal Welfare and Animal Care Committee of the KUPRI and the Animal Research Committee of Kyoto University.
The experimental protocol was reviewed and approved by the Animal Studies Committee of Nihon Bioresearch Inc. and the Animal Research Committee of Morinaga Milk Industry Co., Ltd ,Japan.
The experimental protocol (N. 134/2015-PR) was approved by the Italian Ministry of Health.
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