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In patients where multiple fluid challenges must be repeated in a short time, this inevitably leads to administering a volume of fluid that is far from negligible.
As the HS operation was completed, the animals were resuscitated for 15 minutes by returning all of the shed blood and then administering a volume of Ringer's solution equal to the volume of the shed blood.
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patients who were administered a volume of fluids higher than 5 l of crystalloids or colloids in the preceding 24 h.
Exclusion criteria (to avoid other factors related to IAH) were as follows: patients with abdominal surgery or with suspicion of a surgical abdomen, abdominal distension, ascites, present or recent pregnancy, and abdominal or pelvic trauma and patients who were administered a volume of fluids higher than 5 l of crystalloids or colloids in the preceding 24 h.
Control animals were administered a volume of DMSO corresponding to the volume for pifithrin.
ALC group was orally administered 20% ethanol daily, CUR group was orally fed a 0.016% curcumin (Sigma-Aldrich, Saint Louis, MO, USA) in diet after administering 20% ethanol [ 24, 25], and CON group was administered a volume of saline (0.85% sodium chloride) as equal to that of the alcohol administered to the other 2 groups.
In mice, drugs were administered in a volume of 10 mL/kg; amphetamine and oxotremorine were administered SC, while MK-801 was administered IP.
The CB2 agonist was dissolved in 100% Dimethylsulfoxide (DMSO) as vehicle and administered in a volume of 1 ml/kg bodyweight [31] 60 minutes before the experimental tests, and 4 hours after NTG (or saline+alcohol +propylene glycol) administration.
All these drugs were administered in a volume of 0.1 ml/10 g.
Vaccines were administered in a volume of 10 µl on days 0, 3, 14, 17, 28 and 31 (total of 6 doses).
ASON and NSON were dissolved in sterile saline and administered in a volume of 100 µl containing 4 nmol oligonucleotides intraperitoneally at the day prior to ischemia and directly into the abdominal cavity after release of the clamps.
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