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A second enzymatic digestion using collagenase IV (1 mg/ml), DNase I (2mg/ml), hyaluronidase (1.5mg/ml), and trypsin (1mg/ml) at 37°C for 30 min. was administered to isolate germ cells and Sertoli cells from the remaining tissue.
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When administered to isolated NPCs, CORT-treated ACM increased the percent of BrdU+ NPCs significantly over EtOH-treated coculture media (CoC) control by 1.52-fold (29.56 ± 2.81 CoC-EtOH vs 44.93 ± 2.82 ACM-1 μM CORT; Figure 5C,D).
Isolated heart: Drugs were administered to the isolated heart by intracoronary infusion through the aortic cannula using a syringe pump (KD Scientific, model KDS120).
All ten P. multocida isolates administered to the rabbits were shown to be sensitive to enrofloxacin in the disk diffusion test.
To verify whether hSC seeded on Perimaix released factors that are supportive to neurite outgrowth, conditioned medium was collected from hSC seeded on Perimaix and administered to primary sensory neurons isolated from rat DRG.
Treatment was administered to excised rat hearts and isolated ventricular myocytes via acute perfusion.
However, the beneficial potential of adoptively transferred T cells turned out to be more pronounced if autologous tumor infiltrating lymphocytes (TIL) instead of isolated CTL clones were administered to patients, an effect that was attributed to tumor-specific CD4+ T cells present among the TIL infused [8] [10].
However, when CnA-AID was depleted in mature schizonts and isolated merozoites were intravenously administered to naive mice, we observed a nearly 90% reduction in erythrocyte invasion.
Merozoites were isolated by filtration and intravenously administered to mice.
[ 21] carried out an experimental infection where M. a. paratuberculosis isolated from Norwegian goats was administered to cattle.
Lactobacillus strains isolated from BB-DR rats were administered to BB-DP rats to analyze their effect on T1D development.
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