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First, we needed to administer relatively high doses of agents that were less cytotoxic to cancer cells.
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In general, the tracer doses administered in rodent models must be kept low while at the same time administering relatively high activity doses (> 10 MBq/animal).
In the first set of experiments, we administered relatively high doses (3,600, 720, or 144 pmol/mouse) and performed analyses after 1 hr.
Specifically, in our study with rhesus monkeys, we were required to administer a relatively high (400 μg/kg) dBPA oral dose compared with predicted human BPA oral exposure of < 1 μg/kg/day to achieve serum concentrations similar to those reported in biomonitoring studies.
This protein suffers from very low bioavailability and therefore has to be administered in relatively high doses, which can cause potentially serious side effects like internal bleedings.
In order to demonstrate the functionality and extended pharmacokinetic profile of the molecule, a dose of Gcg-XTEN was administered at relatively high dose (12 nmol/kg) where an increase in blood glucose levels was expected.
For example, antibodies and other binding scaffolds usually need to be administered at relatively high concentrations in order to generate a suitable response in patients.
Dose is an important factor in drug poisoning and most drugs cause clinical signs of poisoning when administered at relatively high dose [ 26].
Additionally, other data indicate that antisense DNA exhibits efficacy in vivo when administered at the relatively high concentration of 5 mM (Whitesell et al. [1991]).
Since the administered doses were relatively high, it seems unlikely that NAC might be a valuable option for the prevention of post-endoscopic esophageal stricture.
administered into tumor at a relatively high temperature and automatically formed a hydrogel after cooling to body temperature.
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