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Supplementary boluses of 3 to 5 μg/kg of fentanyl and 0.1 to 0.5 mg/kg of midazolam were administered as necessary.
Supplements of the ketamine/xylazine cocktail (10% of original dose) were administered as necessary to maintain the animal's level of anaesthesia.
In both groups, dobutamine and fluid challenges were administered as necessary to optimize oxygen delivery.
Sodium bicarbonate was administered as necessary for maintenance of arterial pH at 7.4 ± 0.1.
Blood transfusions were administered as necessary to maintain the hemoglobin concentration at ≥ 9.0 g/dl.
Additional hydration, frusemide (i.v. or oral), potassium, and magnesium supplements were administered as necessary.
Additional anesthesia (0.5 1.0% halothane mixed with 50% nitrous oxide and 50% oxygen) was administered as necessary.
Heparin (250 U/h) was administered as necessary to avoid clotting in the extracorporeal circuit of the dummy device.
In group B concomitant heparin was administered as necessary to achieve an aPTT of approximately 60 seconds.
Mice (30 40 gm) were anesthetized by intraperitoneal injection of pentobarbital (30 mg/kg), and supplemental pentobarbital (10 mg/kg) was administered as necessary to maintain surgical anesthesia.
Even though coagulation factors were administered as necessary the patient suffered from smaller bleedings without clinical relevance in the area of the surgical wound.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com