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The down regulation of those three transcripts can be seen as an adjustment to low temperature as has been observed for many genes involved in energy metabolism.
There are indications that introns pose hindrance to rapid gene regulation and are selected against in those genes which require quick adjustment in transcript level to overcome the environmental challenges [ 43].
Separate ANOVAs were conducted to identify genes whose transcript levels responded significantly (P ≤ 0.01 after step-down Bonferroni adjustment) to each treatment.
This gradual adjustment to conditions is acclimatization.
There is no adjustment to make here.
Feature counts were normalized using the RPM (read per million aligned reads) method, and no adjustment to gene/transcript size was made because our protocol has a limited coverage of 0.5 3 kb from the 3′ end of the transcripts.
Our analysis provided not only information on the cell-specific activity of genes known from transcriptome studies using pooled tissue samples, but moreover identified the differential expression of novel genes and revealed a fine-tuned adjustment of transcript accumulation within root tissues in response to fungal colonization.
To this end we investigated the first step of regulation by adjustment of transcript levels, that represents the basis for translation, modification and ultimately signaling output.
If sequence, as well as sequence context, introduces subtle adjustments to the final measured value of a transcript, then it may not be possible to know which measurement is the most accurate measurement of transcript abundance.
The most significant changes in MeV's architecture have been adjustments to its data model that allow loading of read counts, normalized transcript expression levels, transcript lengths and read library sizes.
All five researchers then reviewed the categories and theme against the original transcripts, which resulted in minor adjustments to the labelling and the order of categories.
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