In the case of safety biomarkers, we wanted to retain the null hypotheses, therefore the use of a more stringent Bonferroni p-value adjustment threshold (p-value <0.0023) for testing 22 independent immune markers would likely be non-conservative, resulting in false negatives.
Therefore, adjustment threshold for calling a mutation and increasing the read depth in samples is required for each gene and can improve consistency.
No human involvement is required except for the adjustment of thresholds in the beginning, which is conducive to the automation of measurement.
Results show that the proposed system does not require a thorough adjustment of thresholds such as in other approaches, since the reference cell profiles automatically includes the particular characteristics of each network.
By combining statistics and manually interactive adjustment, the thresholds of silt contents for categorizing different degraded lands were determined as 3.5% and 8.8%, and it can effectively detect the degraded areas from others; total classification accuracy of this approach reached 86.21%.
To produce relative weights, stepwise logistic regression was done for each participant with adjustment of thresholds for retention of predictors - if necessary - until a solution was found in the model where regression coefficients did not have high standard errors.
Without any adjustment, a threshold of 5.2% was calculated; with adjustment for age, sex, and blood pressure, the threshold was 5.6%, and after a more comprehensive adjustment, the cutoff was 6.0%.
Image processing was done with Photoshop version 7.01, and with ImageJ 1.33 (NIH, public domain, U.S.A .. Image processing included the following steps:(i) correction of tonal value including adjustment of threshold and gamma value, (ii) noise suppression with low pass filters, (iii) sharpening through overlay of highpass (Laplace) and bandpass filtered original images.
To distinguish the cells from the background, contrast adjustment and thresholding were carried out on the MIPs.
A weakness of the algorithm proposed in our study is the need for manual adjustment of threshold levels before starting the batch analysis.
Boolean searches allow for complex query formulation, and are thus well suited to the exhaustive searching necessary for systematic reviews, but they allow no adjustment of threshold for retrieval based on probability or relevance [ 6].
