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The sequences were assigned quality scores based on Newbler consensus q-scores with modifications to account for overlap redundancy and to adjust inflated q-scores.
Cell isolation from the collected larvae largely followed Zhang et al. (2011) with modifications to allow isolation of larger numbers of cells, which included adjusting volumes, incubations, amount of mechanical force by pipetting, and filtering of debris after dissociation.
Dr. Rose said that the rate of complications was expected to fall with modifications to the device.
They presented a new agreement on 12 November, with modifications to more than 50 points.
Mettia has you covered with modifications to each move.
The suggested high-throughput extraction protocol may be adjusted by slight modifications to handle other tissues.
Individual neuron labeling with the iTRAQ reagents multiplex kit (Applied Biosystems, Foster City, CA) was performed essentially according to the manufacturer's manual but with several modifications to minimize sample handling and to adjust for the small sample volumes.
The perspective of the economic analysis is that of the healthcare system and is based on the program budget with the following modifications to adjust for their economic value as opposed to financial costs.
For example, the software could be rapidly adjusted to changes of the underlying database structure with modifications just to the set of SQL commands.
They come up with "mods," modifications to bypass some obstacle or create some new effect.
Global analysis of transcriptome variations associated with a 48 h exposure of mosquito larvae to low doses of insecticides and pollutants revealed their ability to adjust to modifications of their chemical environment.
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