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These results demonstrate that the rate of the reaction can be manipulated by controlling the spatial organization of product and substrate on the monolayer and by adjusting the affinity of the phosphopeptide for the SH2 domain.
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Consequently, evolution can adjust the affinity, specificity and selectivity of each domain-motif interaction in the network [ 10, 47– 47].
The introduction of well-characterized mutations into RBD was used to adjust the affinity and hence the blocking potency of MSOR towards activated Ras.
The fact that Typha adjusts the affinity for P uptake and root biomass more than Cladium and that Typha has thinner root laterals than Cladium [ 15], increases this species' capacity to extract P from low P solutions relative to Cladium.
Therefore, Bolstad et al. [ 9] proposed the GCRMA to adjust the affinity among nucleotides because of different binding strengths between GC and AT rather than the RMA provided by the Affymetrix Console, as the latter is designed for processing Affymetrix chips.
Alternating LNA/DNA monomers were previously shown to improve the probe stability for intracellular detection and adjust the binding affinity.
Fragments A to E were screened at concentrations adjusted to the affinity of each fragment in duplicate.
As mentioned before, the affinity of the individual RBD modules for Ras-GTP is one major parameter that allows adjusting the strength of binding and inhibition.
IFA was used to detect subcellular localization of proteins and to adjust the functional concentration of the affinity-purified antibodies for use in ADCI assays, as described elsewhere [12].
Random mutagenesis is a powerful tool that can be used to create diversity from which mutants with improved binding can be selected in a manner similar to that exploited by the immune system for adjusting antibody affinity and/or specificity against a given antigen.
In turn, these ADP binding affinities were used to adjust the parameter values of a computational model of ANT1 kinetics to explore the potential functional consequences of the acetylation of Lys23 on adenine nucleotide flux through ANT1.
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