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The protein-containing supernatant was then quantified using the microBCA assay (Thermo Scientific) and adjusted to equivalent concentrations prior to analysis.
The protein-containing supernatant was then quantified using the microBCA assay (Thermo Scientific) and adjusted to equivalent concentrations prior to loading on 4 12% polyacrylamide gels (Criterion, BIO-RAD).
Cells were adjusted to equivalent OD600 values, washed and serially diluted, and 10 µl spots of the 10−4, 10−2 and undiluted cultures were plated on LB agar supplemented with increasing levels (1 mM to 15 mM) acrylate.
R. sphaeroides strains J446 and J467 were grown overnight in complete media supplemented with 1 mM acrylate, adjusted to equivalent OD600 values of 0.8, washed three times and resuspended in 1 ml M9 minimal media containing 10 mM succinate, in 25 ml Sterilin universal tubes.
Protein content was measured by the BCA protein assay (Pierce) and samples adjusted to equivalent protein concentrations with sterile water.
The activity of each virus sample was titrated, by assaying serial twofold dilutions, and virus suspensions were adjusted to equivalent NA activities, which fell in the linear portion of the activity curve.
Similar(53)
All meters provided plasma equivalent results except one, which reported whole blood glucose that was adjusted to plasma equivalent values.
The threshold depends on the sensitivity of the method employed, and is adjusted to be equivalent across all methods used to analyse DNA profiles.
When stimulus strength was adjusted to induce equivalent levels of TSSP, no differences in activation of pain-related brain regions occurred across NC and FM groups.
For the subsequent neuraminidase inhibition studies, the concentration of the virus was adjusted to an equivalent of half-maximal neuraminidase activity.
1 ng/ml recombinant human TGF-β or a diluted T cell culture supernatant adjusted to the equivalent TGF-β activity determined by the 293T-caga-Luc-CD32-CD86 TGF-β bioassay was added to 293T-caga-Luc-CD32-CD86 cells for the indicated time, and the cell lysates were run on SDS-PAGE and blotted with anti-phospho-Smad2 antibody.
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