Brazilin and MTX were dissolved in a small volume of dimethylsulfoxide (DMSO) and its concentration was adjusted to working concentration with 0.9% saline for animal administration.
All DNA concentrations were adjusted to a concentration of 50 ng/μl, in a final volume of 5 μl per sample.
All three C. difficile strains were adjusted to equal concentration by optical density measurement.
Concentration of the library was determined using Agilent's QPCR NGS Library Quantification Kit and adjusted to final concentration of 10 nM.
Thereafter, the cells were resuspended with 500 μl binding buffer and adjusted to a concentration of 1 × 106 cells/ml.
Lighting and sound were adjusted to encourage concentration, and participants were each brought behind medical privacy screens to an examination area, with brainwave data displayed on a screen facing them.
HeLa cells were extensively washed in PBS, counted, and adjusted to a concentration of 107/mL.
The solutions were adjusted to a concentration of 0.5 mg/ml and heated to 95°C at a rate of 1°C/min.
For apoptosis assays, Jurkat cells were adjusted to a concentration of 1 × 106 cells/ml in 2 ml of HBSS.
Wild type and mutant sperm were collected as explained and adjusted to a concentration of 5 10×106 cells/ml in HTF.
