Synthetic chaplin peptides were dissolved at a final concentration of 0.5 mg.ml−1 in water and the pH adjusted by titration of NaOH/HCl.
The pH was adjusted by titration with DCl.
The pH was adjusted by titration with either DCl or NaOD.
Protein and dye are then added to this solution, with the concentration of the dye adjusted by titration to give an absorbance which is approximately equal to that of the highest absorbance band in the protein spectra.
Constant pH was adjusted by continuous titration with sodium hydroxide (0.1 N).
The pH of the exchange medium was adjusted by slow titration of 1.0 mol/L NaOH until pH 8.0 was achieved.
Doses were adjusted by forced titration to reach maximum tolerated doses (see web appendix 1).
If the patients required oxygen, the oxygen flow was maintained at their baseline until completion of testing or adjusted based on titration on exertion during home oxygen evaluation.
ST was measured by an internationally accepted, empirical, age-adjusted, titration method at the 1st (IST), 6th, 12th, 18th, and 24th (ST6 12 18 24, respectively) ECT session [ 3].
Sodium acetate (Merck, Darmstadt, Germany) was dissolved in distilled water (Versol, Lyon, France) to a final concentration of 0.1 M, and the pH was adjusted to 5.5 by titration with 1 M HCl (Merck, Darmstadt, Germany).
The pH value of the solution was adjusted to 14 by titration of diluted NaOH, and 0.15 g PVP as surfactant was dissolved in the solution under vigorous stirring.
