Sentence examples for adjusted intensity from inspiring English sources

(c) The adjusted intensity image.

The median intensity value of the background pixels of each image was subtracted from the intensity value of every pixel in that image, and these adjusted intensity values were then truncated at zero.

In each plate, the difference between the adjusted intensity of an shRNA and the median in the sample field was calculated [29].

P-values were calculated by conducting a one sample t-test on the adjusted intensity data of the entire array of selected hybridizations at each time point.

T-tests (calculated by conducting a one sample t-test on the adjusted intensity data of the entire array of selected hybridizations at each time point) were performed between samples to assess reproducibility and similarity.

Since the genes identified by these methods (as being differentially expressed) did not necessarily have expression ratios at all the time points (some were filtered out at one stage, but were retained at another) we manually extracted the adjusted intensity values at these time points and calculated the ratios from the corresponding hybridization data.

The ratio of the resulting adjusted intensities of Cy5 to Cy3 was computed for each spot.

Flagged spots and spots for which background adjusted intensities were < 0 or > 65 000 were excluded from further analysis.

For assessing differential expression, the normexp background correction method with the parameter offset = 50 was applied to each array and resulted in strictly positive adjusted intensities.

Spots with adjusted intensities lower than half the background were manually raised to half the background to avoid extreme expression ratios.

First, background adjusted intensities were log-transformed and the differences (M) and averages (A) of log-transformed values were calculated as M = log2(×1) − log2(×2) and A = [log2(×1) + log2(×2)]/2, where ×1 and ×2 denote the Cy5 and Cy3 intensities, respectively.