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Thereafter, aqueous ammonia was added into the solution to adjust to a pH of 11.
AgNPs were spiked into the prepared bacteria solution to adjust to a constant bacteria concentration of 107 CFU/ml with different AgNP concentrations.
In each set of experiments, the pH of each of the solutions was adjusted to a constant value using solutions of NaOH or HC1.
For preparation of bacterial inocula, colonies from overnight culture of each LAB strain were suspended in 5 mL 0.85% NaCl solution, adjusted to a turbidity of 0.2 ± 0.02 (OD620 nm), and diluted 1 : 500 in LSM broth.
An oil-in-water emulsion was stabilized with a gelatin acacia solution, and the pH of the solution was adjusted to a selected value with acetic acid.
Citric acid (C6H8O7) in 1 1 molar ratio with respect to the metal nitrates was added to the solution as a complexant, and the solution was adjusted to a PH value of 6.5 to 7 by adding ammonia.
Bacterial cells were harvested by centrifugation at 10,000×g at 4 °C for 5 min, then washed twice with saline solution and adjusted to a final concentration of bacteria (9.00 log CFU ml−1) in 2% (v/v) glycerol solution.
This solution was adjusted to a final concentration in the interval 0.1 1 mM before experimental steps.
The pH value of the impregnating solution was adjusted to a specific value by the addition of HCl (37%).
Next, the temperature of the solution was adjusted to a constant 90 °C for 60 min while water was added continuously.
A high voltage of 20 kV was supplied by a direct-current power supply and the feeding rate for the precursor solution was adjusted to a constant rate of 0.3 mL h−1 using a syringe pump.
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